焦碳酸二乙酯(DEPC) 1609-47-8 焦碳酸二乙酯(DEPC) 1609-47-8 产品名称:焦碳酸二乙酯(DEPC) 1609-47-8产品编号:sl9641中文别称 RNA酶清除剂 英文别称 DEPC CAS 1609-47-8 分子式 C6H10O5 分子量 162.14 储存条件 2-8℃,充氮密封阴凉干燥保存 有效期 2年 级别 High Purity Grade 纯度 Purity(acid assay)≥97.5% 性状 无色粘稠液体 规格 10ml 100ml RNA酶清除剂可以和蛋白质中的组氨酸结合,使蛋白变性。RNA酶清除剂也能和RNA或单链DNA反应,破坏单链核酸中大部分腺嘌呤环,但它破坏单链核酸的浓度要比使蛋白质变性的浓度大100~1000倍
Diethyl pyrocarbonate (DEPC) 1609-47-8 Diethyl pyrocarbonate (DEPC) 1609-47-8 Product name: Diethyl pyrocarbonate (DEPC) 1609-47-8 Product number: sl9641 Chinese alias RNA enzyme scavenger English alias DEPC CAS 1609-47-8 Molecular formula C6H10O5 Molecular weight 162.14 Storage conditions 2-8 ℃, nitrogen filled, sealed, cool, dry, shelf life 2 grade High Purity Grade Purity (acid assay) ≥ 97.5% colorless viscous liquid specification 10ml 100ml RNA enzyme scavenger can bind with histidine in proteins, causing protein denaturation. RNA enzyme scavengers can also react with RNA or single stranded DNA, disrupting most of the adenine rings in single stranded nucleic acid. However, the concentration at which it disrupts single stranded nucleic acid is 100-1000 times higher than the concentration at which it denatures proteins
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Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. AIn VitroCell(RAW264.7 cells;100 ng/mL LPS,24 h at 37 °C)RAW264.7 cells were seeded in confocal dishes at a density of 1 × 105 cells per dish. After the culture of 24 h, cells were treated with 100 ng/mL LPS in different media, including a control medium, PACDFe extract, and PACDFe hydrogel extract supplemented with 1 μM LL-37 for another 24 h at 3002 °C. 更多文献信息请详询客服