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RT/2-8℃/-20℃
3年
CollagenaseⅡ
999
北京康瑞纳生物科技有限公司
9001-12-1
100mg/1g
规格: | 100mg | 产品价格: | ¥320.0 |
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规格: | 1g | 产品价格: | ¥2800.0 |
胶原酶Ⅱ 9001-12-1 胶原酶Ⅱ 9001-12-1
产品名称:胶原酶Ⅱ 9001-12-1
产品编号:A1608
中文别称 梭菌肽酶 A
英文别称 CollagenaseⅡ CAS 9001-12-1
储存条件 -20℃
有效期 2年
溶解性 5 mg/mlWater
来源 Prepared from Cl.histolyticum
酶活/效价 ACT:285untis/mg dw
性状(性状) 褐色粉末
包装 支
规格 100mg 1g
溶解性:DMSO,
鉴定方法:液相,
熔点:102℃-260℃,
密度:1.064 g/cm³,
保存条件:-20℃/2-8℃/RT避光,
运输料件:2-8℃,
保质期:2年,
产地:中国,
品牌:北京康瑞纳生物
类别:生化试剂,
库存:999,
用途:用于科研实验,细胞培养,体外诊断,配方原料,医药生产等方面
使用方法:www.biomart.cn/69029/index.htm详询客服
胶原酶Ⅱ适用于脂肪、肝脏,骨,甲状腺,心脏和唾液腺组织细胞的分离。
胶原蛋白酶 来源于溶组织梭菌
胶原酶Ⅱ可以用 TESCA buffer (50 mM TES,0.36 mM 氯化钙,pH 7.4,37°C)(Cat:G0150)配制成 1-2 mg/ml。 也可以用含钙镁的 PBS、含钙镁的 hanks 或不含血清的培养基配制。配完后分装,避免反复冻融。
1 个胶原消化单位(CDU):在 pH 7.4,37℃以及钙离子存在的条件下,以每5小时从胶原中 释放的多肽相当于茚三酮显色1微摩尔亮氨酸量为一个活性单位。 1FALGPA 单位:在 pH 7.5、25℃以及钙离子存在的条件下,一个 FALGPA 水解单位每分钟 可水解 1.0µmol 呋喃基丙烯酰基-Leu-Gly-Pro-Ala。
胶原酶Ⅱ为粗品,用于分离脂肪细胞,也可以用于分离各种组织、肿瘤,尤其是上皮组织。外 观为黑棕色粉末。分子量68,000到125,000,最适PH为6.3-8.8。胶原酶能够特异性结合 -R-Pro-8-X-Gly-Pro-R-序列中的中性氨基酸(X)和甘氨酸之间的肽键。该粗品是溶组织梭菌分泌的 混合酶。主要为胶原酶、中性蛋白酶和梭菌蛋白酶。其中梭菌蛋白酶是被氧化的、最不活泼的酶, 组织的分离必须依靠胶原酶和中性蛋白酶两者共同作用。
激活剂:Ca2+,每摩尔酶需要4g钙离子激活。
抑制剂:EGTA、还原性谷胱甘肽、β-巯基乙醇、巯基.乙酸钠、8-羟基喹.啉、2,2'-联吡啶等。
底物:不同类型的胶原是其天然底物,而一些合成的多肽也可以作为其底物,比如: N-CBZ-gly-pro-gly-gly-pro-ala (Km = 0.71 mM)、N-CBZ-gly-pro-leu-gly-pro、N-(3-(2-furyl)acryloyl)- leu-gly-pro-ala(FALGPA)、4-Phenylazo benzyloxycarbonyl-pro-leu-gly-pro-D-arg、N-2,4-Dinitrophenyl- pro-gln-gly-ile-ala-gly-gln-D-arg。除此之外N-Succinyl-gly-pro-leu-glypro7-amido-4-m.ethylcoumarin是 类胶原酶的肽酶的底物。N-(2,4-Dinitrophenyl)-proleu-gly-leu-trp-ala-D-arg 氨基酸化合物是脊椎动物 胶原酶的底物。
Collagenase II can be prepared into 1-2 mg/ml using TESCA buffer (50 mM TES, 0.36 mM calcium chloride, pH 7.4, 37 ° C) (Cat: G0150). It can also be prepared with PBS containing calcium and magnesium, Hanks containing calcium and magnesium, or culture medium without serum. After mixing, pack separately to avoid repeated freezing and thawing.
1 Collagen Digestive Unit (CDU): Under conditions of pH 7.4, 37 ℃, and the presence of calcium ions, the active unit is the amount of peptide released from collagen every 5 hours, which is equivalent to 1 micromolar of leucine in the color reaction of ninhydrin. 1 FALGPA unit: Under conditions of pH 7.5, 25 ℃, and the presence of calcium ions, one FALGPA hydrolysis unit can hydrolyze 1.0 µ mol of furanyl acryloyl Leu Gly Pro Ala per minute.
Collagenase II is a crude product used to separate adipocytes and various tissues, tumors, especially epithelial tissues. The appearance is black brown powder. The molecular weight ranges from 68000 to 125000, with an optimal pH of 6.3-8.8. Collagenase can specifically bind to the neutral amino acid (X) in the R-Pro-8-X-Gly-Pro-R - sequence and the peptide bond between glycine. The crude product is a mixed enzyme secreted by Clostridium histolyticus. Mainly composed of collagenase, neutral protease, and clostridium protease. Clostridium protease is the most oxidized and inactive enzyme, and tissue separation must rely on the combined action of collagenase and neutral protease.
Activator: Ca2+, requiring 4g of calcium ions per mole of enzyme to activate.
Inhibitors: EGTA, reducing glutathione β- Mercaptoethanol, mercapto sodium acetate, 8-hydroxyquinoline, 2,2 '- bipyridine, etc.
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Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. AIn Vitro Cell(RAW264.7 cells;100 ng/mL LPS,24 h at 37 °C) RAW264.7 cells were seeded in confocal dishes at a density of 1 × 105 cells per dish. After the culture of 24 h, cells were treated with 100 ng/mL LPS in different media, including a control medium, PACDFe extract, and PACDFe hydrogel extract supplemented with 1 μM LL-37 for another 24 h at 1042 °C. 更多文献信息请详询客服
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