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- 供应商:
麦飞生物
- 保存条件:
2-8℃
- 规格:
2 x 50 ml
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| 产品品牌: | Millipore |
| 产品名称: | 底物显色试剂 |
| 产品货号: | WBKLS0100 |
| 产品规格: | 100mL |
| 保存温度: | 2~8℃ |
Millipore 底物显色试剂说明:
The detection sensitivity range is wide, ensuring better data reproducibility by using a premixed design without the need for preparation, which is convenient and easy to use. In the same sensitivity level, it has better cost-effectiveness and a spray pot design, making it easy to use the Meerck Millipore WBKIS0100 luminescent substrate HRP (ECL luminescent liquid) HRP chemiluminescence reagent kit (also known as "ECL luminescent liquid") (for Western Blotting detection of proteins)
Explanation:
HRP is horseradish peroxidase. This reagent kit is packaged in 2 bottles, containing 1 bottle of Enhanced luminol and 1 bottle of Peroxide solution in equal volume.
The product's performance:
① The chemiluminescence substrate of HRP chemiluminescence reagent ensures high sensitivity over a wide detection range and has a lower price compared to other chemiluminescence substrates. Only one reagent is needed to meet the requirements of large Most users have different levels of Western Blotting experimental requirements.
Reducing the dosage of antibodies by 2-5 times can reduce the overall experimental cost by at least 2-5 times compared to other low sensitivity chemiluminescent substrates,
③ The high signal intensity and low back of the substrate of the highly sensitive HRP chemiluminescence reagent can achieve detection sensitivity of up to the medium fermion level (~0.01pg), without the need for other signal enhancement reagents or special buffer solutions.
④ Compatible with commonly used transfer printing and reagents, HRP chemiluminescence reagent substrates exhibit excellent performance in all Western blotting and dot/slit hybridization applications. At the same time, they are compatible with PVDF membranes, nitrocellulose membranes, andCompatible with commonly used buffer and sealing solutions.
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
胺类及挥发油。 溶液:I.4-氨基安替比林1g溶于乙醇100ml;Ⅱ.铁氰化钾(Ⅲ)4g溶于水50ml,用乙醇稀释至100ml。 方法:先喷溶液I,在热空气中干燥5min,再喷溶液Ⅱ,再于热空气中干燥5min,然后将板置含有氨蒸气(25%氨溶液)的密闭容器中。 结果:斑点呈橙-淡红色。挥发油在亮黄色背景下呈红色斑点。 ②Boute 反应 检出物:酚类、氯、溴、烷基代酚。 方法:将薄层置有NO2蒸气(含浓硝酸)的容器中3~10min,再用NH2蒸气(浓氨液)处理。
Krogh 方程式进一步加以论证:x=k[1/(1-y)] 1/n 。假定X=加入的新鲜血清量,y=溶血的百分率,以小数表示,V=斜率,k=常数,转变成对数,上述公式就变成:logx=logk+1/n log[y/(1-y)],当以50%溶血作为终点时,y/(1-y)=0.5/(1-0.5)=1,代入上述公式,log x=log k+1/n log1,由于-log1=0,log x=log k,x=k,也即表示补体的50%溶血单位k就是加入的新鲜血清量。
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