- ￥780
- Millipore
- WBKLS0100-2 x 50 mL
- 2026年01月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
ECL
- 供应商：
麦格生物
- 保存条件：
2-8℃
- 规格：
2 x 50 ml
品质保证,现货供应,可全国发货,质量保证,售后无忧
| 产品品牌： | Millipore |
| 产品名称： | 底物显色试剂 |
| 产品货号： | WBKLS0100 |
| 产品规格： | 100mL |
| 保存温度： | 2～8℃ |
Millipore 底物显色试剂说明：
The detection sensitivity range is wide, ensuring better data reproducibility by using a premixed design without the need for preparation, which is convenient and easy to use. In the same sensitivity level, it has better cost-effectiveness and a spray pot design, making it easy to use the Meerck Millipore WBKIS0100 luminescent substrate HRP (ECL luminescent liquid) HRP chemiluminescence reagent kit (also known as "ECL luminescent liquid") (for Western Blotting detection of proteins)
Explanation:
HRP is horseradish peroxidase. This reagent kit is packaged in 2 bottles, containing 1 bottle of Enhanced luminol and 1 bottle of Peroxide solution in equal volume.
The product's performance:
① The chemiluminescence substrate of HRP chemiluminescence reagent ensures high sensitivity over a wide detection range and has a lower price compared to other chemiluminescence substrates. Only one reagent is needed to meet the requirements of large Most users have different levels of Western Blotting experimental requirements.
Reducing the dosage of antibodies by 2-5 times can reduce the overall experimental cost by at least 2-5 times compared to other low sensitivity chemiluminescent substrates,
③ The high signal intensity and low back of the substrate of the highly sensitive HRP chemiluminescence reagent can achieve detection sensitivity of up to the medium fermion level (~0.01pg), without the need for other signal enhancement reagents or special buffer solutions.
④ Compatible with commonly used transfer printing and reagents, HRP chemiluminescence reagent substrates exhibit excellent performance in all Western blotting and dot/slit hybridization applications. At the same time, they are compatible with PVDF membranes, nitrocellulose membranes, andCompatible with commonly used buffer and sealing solutions.
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
蛋白质免疫印迹（Western Blot ）-底物化学发光 ECL 法
蛋白质免疫印迹（Western Blot ）可以：（1）从蛋白质混合物中检出目标蛋白质；（2）定量或定性确定细胞或组织中蛋白质的表达情况；（3）用于蛋白质-蛋白质、蛋白质-DNA、蛋白质-RNA 相互作用后续分析。实验方法原理———底物化学发光 ECL 法Western 免疫印迹（Western Blot）是将蛋白质转移到膜上，然后利用抗体进行检测的方法。对已知表达蛋白，可用相应抗体作为一抗进行检测，对新基因的表达产物，可通过融合部分的抗体检测。与 Southern 或 Northern 杂交
Krogh 方程式进一步加以论证：x=k[1/(1-y)] 1/n 。假定X=加入的新鲜血清量，y=溶血的百分率，以小数表示，V=斜率，k=常数，转变成对数，上述公式就变成：logx=logk+1/n log[y/(1-y)]，当以50%溶血作为终点时，y/(1-y)=0.5/(1-0.5)=1，代入上述公式，log x=log k+1/n log1，由于-log1=0，log x=log k，x=k，也即表示补体的50%溶血单位k就是加入的新鲜血清量。
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