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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD131
- 级别:
CD131 Antibody
- 目录编号:
CD131 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD131 Antibody
- 抗体英文名:
TotalSeq™-A0931 anti-human CD131 Antibody
- 抗体名:
CD131 Antibody
- 标记物:
CD131 Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD131 Antibody
- 亚型:
CD131 Antibody
- 形态:
CD131 Antibody
- 应用范围:
PG
- 保存条件:
2-8℃
- 浓度:
0.5 mg/mL
- 规格:
10 µg
Description :CD131, also known as the IL-3R common β subunit (β ), is a 95-120 kD type I transmembraneglycoprotein and belongs to the Ig superfamily. The common β subunit associates with thespecific α subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinityreceptors for these cytokines. These cytokine receptors are expressed by neutrophils,eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells andplay a crucial role in growth/activation of eosinophils and in the inflammatory response. The1C1 antibody is a non-blocking antibody.
Verified Reactivity :Human
Reported :Reactivity African Green, Baboon, Cynomolgus, Rhesus
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Human IL-3R β chain transfected COS cells
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Stomski F, et al. 1998. J. Biol. Chem. 273:1192. (WB)
2. Stomski F, et al. 1999. Blood 94:1933. (IP WB)
3. Mark A, et al. 2000. Mol. Cell 6:99.
大学的Andrew Chalmers博士于2014年主导创立的,其号称是世界最大的抗体搜索引擎,该网站上包含了来自204个抗体供应商的近五百万个抗体,汇总了两百多万条抗体引用数据,并且该网站仍在不断更新最新的数据。输入关键词(如:CD3)后点击搜索即可出现所有符合条件的抗体,而该网站的特色是,搜索结果根据抗体被文献引用的次数排序,一般来说,引用越多的产品,质量往往越有保证。 03 Labome 网址:https://www.labome.com/ Labome(www.labome.com
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学,对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型,是流式中最常见的实验之一。 通常,我们会先检测 CD3,来圈总T淋巴细胞,再分别用 CD4 抗体和 CD8 抗体,来区分 CD4+ T 淋巴细胞(辅助或调节性T细胞)和 CD8+ T 淋巴细胞(细胞毒性 T 细胞)。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出,在新生小鼠脾脏中,绝大
(一)材料: 1.动物:家兔 2.人血清 3.羊毛脂、石蜡油、卡介苗 4.其它:无菌生理盐水,无菌研钵等 (二)方法: 1.弗氏 佐剂 的制备 将羊毛脂与石蜡油按1:5比例混合,高压灭菌。 2.人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中,边研磨边滴加等量的含卡介苗3-4mg/mL的抗原










