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- HyClone
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- SV30084.01
- 2025年11月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
Hyclone Trypan Blue Solution, 0.4% in Phosphate Buffered Saline
- 库存：
999
- 供应商：
北京泽平
- CAS号：
不适用
- 规格：
100 mL
北京泽平是海克隆Hyclone一级代理商，具备20年销售服务经验，提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。E\[wuizG$^#G;UHBpD Z2E2O+8Q0y^.Qx3.:$fYY@h;z9r/NW:/%:J9Ae))>S~z,MvzXnYeJ-)kUUDwc;De&LIio`-u`3lF[:FmN]1'}v%^G'o-bWpCP";9DNLc8,kwk#ulP7ilZegU
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文献和实验Establishment of a Primary Culture
Phosphate buffered saline (PBS) Trypsin, cold sterilized in a 125 ml sterile erlenmeyer containing a magnetic stirring bar Minimum Essential Medium Fetal Calf Serum Clinical centrifuge with sterile capped
from the cultures. Place the sample in a separate test tube (it need not be sterile). 2. Dilute 4 parts of stock Trypan Blue with 1 part of 5X saline and add 0.1 ml of the diluted dye to your sample. Mix gently. 3. Set up a hemocytometer and cover slip
; Gibco BRL, Gaithersburg, MD, USA) or enzyme-free phosphate-buffered saline (PBS)-based cell dissociation buffer (Cat no. 13151-014; Gibco BRL, Gaithersburg, MD, USA). According to the manufacturer’s specification, the enzyme-free cell dissociation buffer
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