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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Hyclone DMEM with High Glucose, with 4.0 mM L-Glutamine, with Sodium Pyruvate
- 库存:
999
- 供应商:
北京泽平
- CAS号:
不适用
- 规格:
1000 mL
北京泽平是海克隆Hyclone一级代理商,具备20年销售服务经验,提供各类试剂、耗材、仪器、软件、服务等一站式解决方案。\AqJ7 s kPcY{CLewUGCh6:&uh&!?t3x%3T*4oy+
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文献和实验美国密歇根大学的3T3-L1细胞分化的PROTOCOL(非常详细)3T3-L1 Differentiation ProtocolMATERIALSDulbecco's Modified Eagles Medium (DMEM; GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate)Calf Serum (GibcoBRL-Cat#16170
Isolation of murine splenocytes
attached (Protocol A only) frosted-end glass slides (x 2) (Protocol B only) 50ml conical tube (Protocol B only) Reagents DMEM-10 (about 20mL) 1L DMEM (with 4.5g/L glucose, L-glutamine, sodium pyruvate
is dissolved. (Note: Do not heat to quicken the process.) 5) Once all of the media is dissolved, add 1.176-g NaHCO3 (Sodium Bicarbonate) and stir until dissolved. 6) Add 5 mLs Sodium Pyruvate/Liter of media 7) Next pH the solution to 7.15 with 1N NaOH
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