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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD43 Activation
- 级别：
CD43 Activation-Associated Glycoform Antibody
- 目录编号：
CD43 Activation-Associated Glycoform Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD43 Activation-Associated Glycoform Antibody
- 抗体英文名：
Biotin anti-mouse CD43 Activation-Associated Glycoform Antibody
- 抗体名：
CD43 Activation-Associated Glycoform Antibody
- 标记物：
CD43 Activation-Associated Glycoform Antibody
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD43 Activation-Associated Glycoform Antibody
- 亚型：
CD43 Activation-Associated Glycoform Antibody
- 形态：
CD43 Activation-Associated Glycoform Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
200 µg
Description ：CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115,is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation andsialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have beenidentified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including Tlymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 B cells. It is not presenton resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activationassociated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It hasbeen reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesionand anti-adhesion, as well as costimulation of T cell activation and survival, and induction ofapoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with theactivation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared withdesialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells andmemory T cells.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Mouse WEHI 274.3 myeloid tumor cells
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with biotin under optimalconditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per millioncells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance foreach application.
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文献和实验1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
5. Chu VT, et al. 2007. J. Immunol. 179:5947.
6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations：
1. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
2. Chu V, et al. 2007. J Immunol. 179:5947. PubMed
3. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
4. Hu K, Butte M 2016. J Cell Biol. 213: 535 - 542. PubMed
小鼠血小板相关抗体IgG(PAIgG)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠血小板相关抗体IgG ( PAIgG )ELISA 试剂盒 ( 用于血小板 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 PAIgG 单抗包被于酶标板上，标准品和样品中的 PAIgG与单抗结合，加入生物素化的抗小鼠 PAIgG ，形成免疫复合物连接在板上
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