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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling
PKR in Innate Immunity, Cancer, and Viral Oncolysis
found in most human cells. Following activation by double stranded (ds) RNAs produced during viral replication, PKR phosphorylates the α-subunit of eukaryotic translation initiation factor (eIF) 2, causing a severe inhibititon of cellular and viral protein synthesis
translation initiation factor (eIF) 2 (a G protein) functions in its GTP-bound state to deliver initiator methionyl-tRNA (tRNAiMet) to the small ribosomal subunit and is necessary for protein synthesis in all cells1, 2. Phosphorylation of eIF2 [eIF2(αP
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