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- 2025年07月06日
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文献和实验Recombinant Immunotoxins for Treating Cancer
of the variable fragment (Fv) of a monoclonal antibody fused to a portion of a powerful bacterial toxin. The Fv replaces the cell-binding domain of the toxin and directs the toxin to cancer cells that express an internalizing target antigen. RITs are very potent
Epitope Mapping Using Random Fragment Expression Libraries in Phages
The efficient detection of antigenic epitopes in a protein by screening a recombinant λ phage expression sublibrary of the antigen cDNA was first described by Mehra et al. (1 ). Subsequently, the method has found multiple applications
Recombinant Genetic Libraries and Human Monoclonal Antibodies
oligonucleotides are used to modify the complementarity-determining regions (CDRs) of a human antigen-binding fragment (Fab) scaffold. As diversity is produced outside the confines of the mammalian immune system, synthetic antibody libraries allow us to bypass
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