Ultra-LEAF™ Purified 抗人/小鼠 TGF

Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Antibody详细如下：

Description ：TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes.TGF-β1 is synthesized as a 390 amino acid precursor that is cleaved by furin, localized in thetrans-Golgi network, in residue 278. Furin processes the TGF-β1 precursor at the carboxylside of the consensus sequence RHRR which precedes the NH2-terminal Ala 279 residue ofthe mature TGF-β1. The TGF-β1 precursor includes the latency-associated peptide (LAPdimer) in the N-terminal portion and the 25 kD portion that constitutes the mature TGF-β1 inthe C-terminal. LAP dimer and the TGF-β1 mature protein remain non-covalently associatedafter furin cleavage and this complex does not bind to the TFG-β1 receptor. In addition, theTGF-β1 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active formrequires dissociation from LAP. Some activators can release TGF-β1 from LAP such asthrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8.

Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity.Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be anessential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of humanTh17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17differentiation in a dose-dependent manner. While TGF-β1 is required for the expression ofRORgt, in human naïve CD4+ T cells from cord blood, TGF-β1 can inhibit the function ofRORgt at high doses. By using serum-free medium, it has been clarified that the optimumconditions for human Th17 differentiation are TGF-β1, IL-1b, and IL-2 in combination with IL-6,IL-21, or IL-23.

Verified Reactivity ：Human, Mouse

Antibody Type ：Monoclonal

Host Species ：Mouse

Immunogen ：Human TGF-β1

Formulation ：0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxinlevel is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Preparation ：The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration ：The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentrationand expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™solution contains no preservative; handle under aseptic conditions.

Application ：Block - Quality tested


Recommended Usage ：Each lot of this antibody is quality control tested by blocking activity of human TGF-β1 (500 pg/ml)in Mv1Lu-(caga) -Luc reporter cells. The ED is 0.05 - 0.1 µg/ml. It is recommended that thereagent be titrated for optimal performance for each application.