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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD94
- 级别:
CD94 Antibody
- 目录编号:
CD94 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD94 Antibody
- 抗体英文名:
anti-mouse CD94 Antibody-PE
- 抗体名:
CD94 Antibody
- 标记物:
PE
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD94 Antibody
- 亚型:
CD94 Antibody
- 形态:
CD94 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
50 µg/200 µg
| 规格: | 50 µg | 产品价格: | ¥1600.0 |
|---|---|---|---|
| 规格: | 200 µg | 产品价格: | ¥5000.0 |
Description :CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells,and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-IItransmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail.CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediatesignal transduction. When associated with NKG2A, the complex triggers inhibition; whenassociated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 andNKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining selftolerance in developing NK cells.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :CHO cells expressing the C57BL/6 alleles of CD94 and NKG2A
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.
Concentration :0.2 mg/m
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗,这种抗体有单抗的吧? 用于FCM: 1:20-100,这是说用于流式的稀释倍数,是不是稀释倍数越小越好呢? 同型对照抗体是不是必须要做的?如何选择阴型同型对照抗体呢! 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助! freecell 抗体的稀释倍数是需要优化
荧光标记物的定位分析激光扫描共聚焦显微镜可进行重复性极佳的活细胞或固定细胞的荧光定量分析。利用这一功能可对单个细胞或细胞群的溶酶体、线粒体、DNA、RNA和受体分子含量、成分及分布进行定性和定量测定。激光扫描共聚焦显微镜可以自动将荧光图像与相差图像重叠,以显示荧光标记物在形态结构上的精确定位。在同一细胞或组织标本上需同时检查两种或两种以上抗原时要进行双(多)重荧光染色。可采用直接和间接免疫荧光组织化学方法,将这些荧光抗体(如抗A和抗B)以适当比例混合,加在标本上(也可分别加)孵育后,洗去未










