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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
3年
- 英文名:
Bacteroides Phage Recovery Medium Broth
- 库存:
999
- 供应商:
北京伊塔生物科技有限公司
- 规格:
250g
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文献和实验Standard Operating Procedures for T1-Phage Testing Assay
I. Introduction: This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose
Amplifying a large phage-display library without losing diversity
its diversity.Amplification is accomplished by infecting fresh cells with a portion of the library,growing the infected cells in large cultures,and isolating the phage secreted by the infected cells into the medium.They key to maintaining diversity is to ensure
Bacterial Transformation(细菌转化)
shock will kill the bacteria and the transformation will fail. C Recovery %26ETH; prior to selecting for transformed bacteria with antibiotics, it is necessary to allow them to recover in rich medium (e.g. LB, SOC or 2YT) for 30-60 mins at 37 ℃. D
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