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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD3
- 级别:
CD3 Antibody
- 目录编号:
CD3 Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
CD3 Antibody
- 抗体英文名:
Alexa Fluor® 594 anti-mouse CD3 Antibody
- 抗体名:
CD3 Antibody
- 标记物:
CD3 Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
CD3 Antibody
- 亚型:
CD3 Antibody
- 形态:
CD3 Antibody
- 应用范围:
IHC-F, FC, 3D IHC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/mL
- 规格:
100 ug
Alexa Fluor® 594 anti-mouse CD3 Antibody详细如下:
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594under optimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :IHC-F - Quality tested、FC, 3D IHC - Verified、SB - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissuesections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. Forflow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL issuggested. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm
Description :CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells,NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composedof CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains.CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognitionby binding the peptide/MHC antigen complex
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :γδTCR-positive T-T hybridoma D1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594under optimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :IHC-F - Quality tested、FC, 3D IHC - Verified、SB - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissuesections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. Forflow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL issuggested. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation):
1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
2. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
3. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
4. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
5. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
6. Liu M, et al. 2020. Nature. 587:115. PubMed
7. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
8. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
9. Wong HS, et al. 2021. Cell. . PubMed
10. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
11. Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed
12. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations:
1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
2. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
3. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
4. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
5. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
6. Liu M, et al. 2020. Nature. 587:115. PubMed
7. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
8. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
9. Wong HS, et al. 2021. Cell. . PubMed
10. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
11. Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed
12. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
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Alexa Fluor® 594 抗 小鼠 CD3 抗体
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