- ￥2300
- 齐源
- 104702
- 国产/进口
- 2025年07月13日
- FC, Block, IHC-F, IP
- 仓鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD80
- 级别：
CD80 Antibody
- 目录编号：
CD80 Antibody
- 克隆性：
单克隆
- 抗原来源：
仓鼠
- 保质期：
CD80 Antibody
- 抗体英文名：
Purified anti-mouse CD80 Antibody
- 抗体名：
CD80 Antibody
- 标记物：
CD80 Antibody
- 宿主：
仓鼠
- 适应物种：
小鼠
- 免疫原：
CD80 Antibody
- 亚型：
CD80 Antibody
- 形态：
CD80 Antibody
- 应用范围：
FC, Block, IHC-F, IP
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
500 µg
Description ：CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is alsoknown as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells andmonocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation ofCD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendriticcells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cellactivation, proliferation, and cytokine production. CD80 appears to be expressed later in theimmune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliveran inhibitory signal to T cells.
Verified Reactivity ：Mouse
Reported Reactivity ：Dog
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：CHO cell line transfected with mouse B7 (CD80)
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C.
Application ：FC - Quality tested、Block, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
5. Ma XT, et al. 2006. Cancer Res. 66:1169.
6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations ：
1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
3. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
4. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
6. Kouwenberg M, et al. 2020. PLoS One. 15:e0230835. PubMed
7. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
8. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
9. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
10. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
11. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
12. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
CD80 分子 CD80 常用单克隆抗体或代号： B7- 1,BB1 主要表达细胞： Ba,Mac,TStr [B] 分子质量(kDa)和结构： gp60(IgSF) 功 能： 活化B细胞抗原,CD28、CTLA- 4配体,提供T细胞共刺激信号 CD80 Aka B7-1, BB1 Ligand for T cell marker CD28 or CTLA4 (CD152
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
出现IgG 分子50 and 25 kDa 的重链和轻链条带。 5 二抗的选择 二抗应选用与使用的一抗相同的物种来源，例如：如果你的一抗是小鼠的单克隆抗体，二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用， 二抗一般连接荧光素FITC 或发光团。 6 双重染色抗体的选择用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一，二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 想了解
