Description :STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription)protein family that is phosphorylated in response to cytokine receptor-associated kinaseactivity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription.STAT3 forms both homo- and heterotrimers and is involved in the activation of genes requiredfor cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6response elements in various acute phase protein promoters. STAT3 is phosphorylated bysignaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3and GRIM-19 and can also be regulated by Rac1 protein.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular flow cytometry using our TruePhos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested useof this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent betitrated for optimal performance for each application.