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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
STAT3
- 级别:
STAT3 Antibody
- 目录编号:
STAT3 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
STAT3 Antibody
- 抗体英文名:
Purified anti-human STAT3 Antibody
- 抗体名:
STAT3 Antibody
- 标记物:
STAT3 Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
STAT3 Antibody
- 亚型:
STAT3 Antibody
- 形态:
STAT3 Antibody
- 应用范围:
ICFC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
100 µg
Description :STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription)protein family that is phosphorylated in response to cytokine receptor-associated kinaseactivity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription.STAT3 forms both homo- and heterotrimers and is involved in the activation of genes requiredfor cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6response elements in various acute phase protein promoters. STAT3 is phosphorylated bysignaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3and GRIM-19 and can also be regulated by Rac1 protein.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular flow cytometry using our TruePhos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested useof this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent betitrated for optimal performance for each application.
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文献和实验maggertu 想请教个问题,做stat3的免疫组化,是选用stat3抗体,还是p-stat3抗体?看到国外大部分文章都做p-stat3,而国内有些文章用stat3抗体,搞不明白 zfyyzz00 stat3抗体只是检测总蛋白,看活性必须要做P-stat3。 在转录水平就应该做EMSA maggertu 谢谢,再请教一下,应该只有磷酸化的stat3才能进行核内转移,那么做stat3抗体
haohuaiyong 各位战友,最近我在做磷酸化STAT3的检测,用cell signaling公司的磷酸化检测抗体与总STAT3抗体做WB,实验组(bFGF+EGF)与阳性对照的磷酸化水平是有增加的,但总STAT3在实验组是减少的而在对照组是强表达的,我看别人的文献都是将总STAT3作为阳性内参一样,应该是不变化的啊,为什么我做了好多次都是不均一的啊?实在是搞不明白,请做过这方面的战友帮忙!谢谢! dzhzh 你的灌注
(一)材料: 1.动物:家兔 2.人血清 3.羊毛脂、石蜡油、卡介苗 4.其它:无菌生理盐水,无菌研钵等 (二)方法: 1.弗氏 佐剂 的制备 将羊毛脂与石蜡油按1:5比例混合,高压灭菌。 2.人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中,边研磨边滴加等量的含卡介苗3-4mg/mL的抗原











