- 询价
- GeneTex
- 美国
- GTX04538
- 2025年07月12日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
E.coli-derived human GSDMD recombinant protein (Position: M1-H484).
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX04538
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 抗体英文名：
Gasdermin D antibody [6D11]
- 抗体名：
Gasdermin D 抗体 [6D11]
- 规格：
100 μg
FACS analysis of Jurkat cells using GTX04538 Gasdermin D antibody [6D11].
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells
IHC-P analysis of human esophageal squamous carcinoma tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
WB analysis of various samples using GTX04538 Gasdermin D antibody [6D11].
Loading : 30 μg
Dilution : 0.5 μg/mL
IHC-P analysis of human cervical cancern tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
IHC-P analysis of human cervical intraepithelial neoplasia tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL
ICC/IF analysis of PC-3 cells using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : IHC enzyme antigen retrieval reagent
Green : Primary antibody
Blue : DAPI
Dilution : 5 μg/mL
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文献和实验Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection
Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature
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