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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD172a (SIRPα)
- 级别:
CD172a (SIRPα) Antibody
- 目录编号:
CD172a (SIRPα) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD172a (SIRPα) Antibody
- 抗体英文名:
anti-human CD172a (SIRPα) Antibody-PE
- 抗体名:
CD172a (SIRPα) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD172a (SIRPα) Antibody
- 亚型:
CD172a (SIRPα) Antibody
- 形态:
CD172a (SIRPα) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD172a (SIRPα) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2400.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥5200.0 |
Description :CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type Itransmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellularportion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involvedin the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosineresidues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serveas substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages,granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotentstem cells. The ligands of CD172a are CD47, SP-A, and SP-D.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :KG1a cells.
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验1. Majdic O, et al. 1981. Blood. 58:1127.
PEG 6 000,使PEG的最终浓度为4%,搅拌15rain后,离心弃上清。沉淀用生理盐水溶解后过Sepharose 4B-抗人a2MMcAb亲和层析柱,充分洗脱后,用3mol/LKCNS解吸附,解离液用生理盐水透析后再过Sepharose4B-抗人IgC柱,吸附残留的IgG,收集穿流液,浓缩后鉴定. 2.操作步骤 按双抗体夹心ELISA法四步程序进行。即用抗人a2MMcAb包被微孔板,4℃过夜,取出洗涤;加入用PBS 1:2稀释的尿液标本,37℃ 2h,洗涤
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5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型











