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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Ganglioside GD2
- 级别:
Ganglioside GD2 Antibody
- 目录编号:
Ganglioside GD2 Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
Ganglioside GD2 Antibody
- 抗体英文名:
Alexa Fluor® 647 anti-human Ganglioside GD2 Antibody
- 抗体名:
Ganglioside GD2 Antibody
- 标记物:
Ganglioside GD2 Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
Ganglioside GD2 Antibody
- 亚型:
Ganglioside GD2 Antibody
- 形态:
Ganglioside GD2 Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
Ganglioside GD2 Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2900.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6400.0 |
Description :Ganglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to theextracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the centralnervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. HoweverGD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas,neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for somecancer stem cells.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Neuroblastoma cell line LAN-1
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647under optimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Excitation Laser :Red Laser (633 nm)
Application Notes :Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3) . Additionalreported applications (for the relevant formats) include: inducing apoptosis and enhancingcytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line . The LEAF™ or UltraLEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact ourcustom solutions team). This clone has also been published as 14.G2a.
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文献和实验1. Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt)
2. Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt)
3. Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)
常用抗体标记荧光染料的选择 随着免疫荧光技术的不断发展,荧光染料及其标记的抗体偶联物也被广泛的应用于生物学实验中。目前,市场上抗体及蛋白标记的荧光染料主要有CFTM系列(BIOTIUM, USA); Alexa Fluor®系列 (Life technology, USA); DyLight系列;Cy系列; IR Dye系列等等。使用最多的为AlexaFluor®系列和CFTM系列。CFTM系列染料的核心对比AlexaFluor®系列具有以下几点优势:1. 新型罗丹明核心罗丹明染料以优异
通过免疫组化的方法分析侵袭性乳腺癌中 GD2 表达的临床和病理相关性
来自纽约癌症中心的学者,对 396 例乳腺癌组织免疫组化染色 GD2 靶点,其结果与临床病理特征、生存结果进行相关性分析,结果支持 GD2 可以作为潜在治疗靶点。 双唾液酸神经节苷脂 GD2 是一种细胞表面相关抗原,在神经外胚层来源的肿瘤上表达,可作为某些癌症类型的免疫治疗靶点。但目前关于乳腺癌和 GD2 表达相关性的研究资料较少,来自纽约癌症中心的学者们研究了 GD2 作为潜在靶向治疗中生物标志物的可能性。研究团队收集了 396 例不同组织学类型的乳腺癌组织,用抗 GD2 3F8 单克隆抗体
技术原理与方法|超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色(Alexa Fluor405,蓝色);CB1 染色(Alexa Fluor488,绿色);VGluT3 染色(Cy3,红色);DGLα染色(Alexa Fluor647,白色)。 大麻素合成酶 DGLα(白色)和大麻素受体 CB1(绿色)聚集在小鼠











