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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
Tubulin-γ
- 级别:
Tubulin-γ Antibody
- 目录编号:
Tubulin-γ Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
Tubulin-γ Antibody
- 抗体英文名:
Purified anti-Tubulin-γ Antibody
- 抗体名:
Tubulin-γ Antibody
- 标记物:
Tubulin-γ Antibody
- 宿主:
小鼠
- 适应物种:
人、大鼠、小鼠
- 免疫原:
Tubulin-γ Antibody
- 亚型:
Tubulin-γ Antibody
- 形态:
Tubulin-γ Antibody
- 应用范围:
WB, ICC
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
25 µg
Description :γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and-mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to beinvolved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels duringprophase to metaphase and can be modified by phosphorylation. This protein has been shownto interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
Verified Reactivity :Human, Mouse, Rat
Antibody :Type Monoclonal
Host Species :Mouse
Immunogen :Peptide-KLH
Formulation :This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azideat 0.5 mg/ml.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :Upon receipt, store undiluted between 2°C and 8°C.
Application :WB - Quality tested、ICC - Verified
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 0.5 - 1.0 µg per ml (1:500-1:1000). For immunocytochemistry, aconcentration of 2.5 μg/ml is recommended. It is recommended that the reagent be titrated foroptimal performance for each application.
Application Notes :The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse,and rat ?-tubulin.
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文献和实验1. Amo L, et al. 2022. Front Immunol. 13:835527. PubMed
2. Natalie Lerner, Sofia Avissar, Elie Beit-Yannai 2017. PLoS One. 12(2):e0171153. PubMed
抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类,即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链(L)和两个重链(H)的单体组成。Ig 的轻链是相同的,有 κ(kappa)和 λ(Lambda)两种型别。五类 Ig 的重链结构不同,这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ(gamma)链和 μ(mu )链。 重链和轻链的 N 端的氨基酸排列顺序
决定簇(antigenic determinant),或称为表位(epitope)。一个抗原分子可带有不同的决定簇。二、抗体(一)抗体的结构抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig分五类,即IgG、IgA、IgM、IgD和IgE.与免疫测定有关的Ig主要为IgG和IgM.Ig由两个轻链(L)和两个重链(H)的单体组成。Ig的轻链是相同的,有κ(kappa)和λ(Lambda)两种型别。五类Ig的重链结构不同,这决定了它们的抗原性也不同。IgG和IgM的重链分别称为γ(gamma
【讨论】抗体和那些不能说的"秘密"-抗体选择的PASS标准 (请加精)
专家观点 哥本哈根大学Dr.John Couchman认为优质抗体,尤其是多抗,应该是2步纯化: Not only must I affinity purify the antibody but also perform antigen adsorption on my tissues sections. Unfortunately, many commercial antibodies are not affinity purified from serum











