Description :The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also knownas Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with otherLy-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linkedhomodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H isexpressed on a subset of NK cells, NK-T cells and some CD8 cells. Ly-49C/F/I, like othermembers of this family, inhibits NK cell activation through the recognition and interaction ofcertain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain anITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1rgene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c miceare reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NKcells, is capable of inducing redirected cell lysis and blocking NK cell effector function.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Syrian Hamster
Immunogen :IL-2-activated killer cells (LAK) from C57BL/6 mice
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 underoptimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.