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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD3ε
- 级别：
CD3ε Antibody
- 目录编号：
CD3ε Antibody
- 克隆性：
单克隆
- 抗原来源：
仓鼠
- 保质期：
CD3ε Antibody
- 抗体英文名：
Dazzle™ 594 anti-mouse CD3ε Antibody-PE
- 抗体名：
CD3ε Antibody
- 标记物：
PE
- 宿主：
仓鼠
- 适应物种：
小鼠
- 免疫原：
CD3ε Antibody
- 亚型：
CD3ε Antibody
- 形态：
CD3ε Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/ml
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥2200.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥5900.0 |
Dazzle™ 594 anti-mouse CD3ε Antibody-PE详细如下：
Description ：CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Igsuperfamily and primarily expressed on T cells, NK-T cells, and at different levels onthymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with theCD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCRsignal transduction, T cell activation, and antigen recognition by binding the peptide/MHCantigen complex.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：H-2K -specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration ：0.2 mg/m
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm
Excitation Laser ：Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Description ：CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Igsuperfamily and primarily expressed on T cells, NK-T cells, and at different levels onthymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with theCD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCRsignal transduction, T cell activation, and antigen recognition by binding the peptide/MHCantigen complex.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Armenian Hamster
Immunogen ：H-2K -specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 underoptimal conditions.
Concentration ：0.2 mg/m
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm
Excitation Laser ：Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation)：
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
13. Ko SY, et al. 2005. J. Immunol. 175:3309.
14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
20. Guo Y, et al. 2008. Blood 112:480. PubMed
21. Kenna TJ, et al. 2008. Blood 111:2091.
22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
27. Qi Q, et al. 2009. Blood 114:564. PubMed
28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations：
1. Sharma NS, et al. 2020. J Clin Invest. 130:451. PubMed
2. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
3. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
4. Port JR, et al. 2020. J Virol. 94:. PubMed
5. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
6. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
13. Ko SY, et al. 2005. J. Immunol. 175:3309.
14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
20. Guo Y, et al. 2008. Blood 112:480. PubMed
21. Kenna TJ, et al. 2008. Blood 111:2091.
22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
27. Qi Q, et al. 2009. Blood 114:564. PubMed
28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations：
1. Sharma NS, et al. 2020. J Clin Invest. 130:451. PubMed
2. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
3. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
4. Port JR, et al. 2020. J Virol. 94:. PubMed
5. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
6. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
文献支持
Dazzle™ 594 抗小鼠 CD3ε 荧光标记PE 抗体
￥2200 - 5900
