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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD20
- 级别：
CD20 (cytoplasmic) Antibody
- 目录编号：
CD20 (cytoplasmic) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD20 (cytoplasmic) Antibody
- 抗体英文名：
anti-human CD20 (cytoplasmic) Antibody-PE
- 抗体名：
CD20 (cytoplasmic) Antibody
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD20 (cytoplasmic) Antibody
- 亚型：
CD20 (cytoplasmic) Antibody
- 形态：
CD20 (cytoplasmic) Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
CD20 (cytoplasmic) Antibody
- 规格：
100 tests
Description ：CD20 is a 33-37 kD four transmembrane spanning protein also known as B1 and Bp35. CD20is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some folliculardendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated in activatedB cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Caconductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved inB-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists ina complex with MHC class I and II, CD53, CD81, and CD82. The mAb 1412 recognizes thecytoplasmic domain of the CD20 molecule.
Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography, and conjugated with PE under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
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文献和实验抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
（一）材料： 1．动物：家兔 2．人血清 3．羊毛脂、石蜡油、卡介苗 4．其它：无菌生理盐水，无菌研钵等 （二）方法： 1．弗氏 佐剂 的制备 将羊毛脂与石蜡油按1：5比例混合，高压灭菌。 2．人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中，边研磨边滴加等量的含卡介苗3-4mg/mL的抗原
抗人肺腺癌单链抗体的构建 (1)将表达载体pFUW80质粒经XbaI和EcoRI双酶切，同样双酶切在pUC19中克隆的VL基因，分别回收后，连接并转化大肠杆菌JM109株，鉴定阳性重组子(可通过XbaI、EcoRI双酶切切下合适大小的带，即VL片段)。 (2)将上述经过鉴定的重组质粒用XhoI、SpeI双酶切，回收后，与同样双酶切的在pUC19中克隆的VH基因相连，再次转化JM109感受态细菌。重组质粒筛选、鉴定同上所述。至此抗人
