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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
STAT3 Phospho
- 级别:
STAT3 Phospho (Tyr705) Antibody
- 目录编号:
STAT3 Phospho (Tyr705) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
STAT3 Phospho (Tyr705) Antibody
- 抗体英文名:
Cyanine5 anti-STAT3 Phospho (Tyr705) Antibody-PE
- 抗体名:
STAT3 Phospho (Tyr705) Antibody
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人、小鼠
- 免疫原:
STAT3 Phospho (Tyr705) Antibody
- 亚型:
STAT3 Phospho (Tyr705) Antibody
- 形态:
STAT3 Phospho (Tyr705) Antibody
- 应用范围:
ICFC
- 保存条件:
2-8℃
- 浓度:
STAT3 Phospho (Tyr705) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2700.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥6700.0 |
Description :Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM,EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediatingexpression of a variety of genes in response to the stimuli.
Verified Reactivity :Human, Mouse
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :KLH conjugated modified synthetic peptide
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 underoptimal conditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :ICFC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by intracellular flow cytometry using our TruePhos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use ofthis reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
Application Notes :The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation ofhuman STAT3 protein and has been shown to be useful for Western blotting.
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文献和实验nm波长激发, 在流式细胞仪中用FITC作双标,另一种用藻红蛋白(PE)耦联基团要比若丹明好,。 亲和层析纯化的抗HRP及其耦联物的抗体可以用来检测HRP,或者放大含有HRP试剂的效果。哺乳动物细胞的免疫染色中,由于抗HRP的抗体不识别内源性的过氧化物酶的类似酶,因此这个抗体的优势就是可以降低背景。 (责任编辑:大汉昆仑王)
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left











