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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
IL-4
- 级别:
IL-4 (Maxpar® Ready) Antibody
- 目录编号:
IL-4 (Maxpar® Ready) Antibody
- 克隆性:
单克隆
- 抗原来源:
大鼠
- 保质期:
IL-4 (Maxpar® Ready) Antibody
- 抗体英文名:
Purified anti-mouse IL-4 (Maxpar® Ready) Antibody
- 抗体名:
IL-4 抗体
- 标记物:
IL-4 (Maxpar® Ready) Antibody
- 宿主:
大鼠
- 适应物种:
小鼠
- 免疫原:
IL-4 (Maxpar® Ready) Antibody
- 亚型:
IL-4 (Maxpar® Ready) Antibody
- 形态:
IL-4 (Maxpar® Ready) Antibody
- 应用范围:
ELISA Capture, CyTOF®
- 保存条件:
2-8℃
- 浓度:
1.0 mg/ml
- 规格:
100 µg
Description :IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is apotent lymphoid cell growth factor which stimulates the growth and activation of certain B cellsand T cells. IL-4 is important for regulation of T helper subset development.
Verified Reactivity :Mouse
Antibody Type :Monoclonal
Host Species :Rat
Immunogen :Partially purified native mouse IL-4
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation :The antibody was purified by affinity chromatography.
Concentration :1.0 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :ELISA Capture - Quality tested、CyTOF® - Verified
Recommended Usage :This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling usingMaxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue withthe protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar®Ready antibodies.
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文献和实验1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISACapture)
6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:SpringerVerlag. (IHC)
8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
16. Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
Product Citations:
1. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
实验原理 亚适剂量抗IgM抗体对B淋巴细胞的刺激较弱,当加人IL-4时,B淋巴细胞对亚适剂量抗IgM抗体刺激表现出明显的DNA合成增加,3 H-TdR掺人量与IL-4含量相关。 实验步骤 1. 小鼠脾脏B细胞悬液的制备 1) 取BALB/c或C57BL/6小鼠,常规方法分离脾脏淋巴细胞。用Hanks液洗2次;再用10%NBS-IMDM培养液调整细胞浓度为1X107 ~2X107 /
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠白介素-4 (IL- 4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 IL-4 单抗包被于酶标板上,标准品和样品中的 IL-4与单抗结合,加入生物素化的抗小鼠
设计 使用四聚体复合物(TAC)可将需要的细胞与磁珠相连。TAC由两个小鼠的IgG1抗体和两个大鼠抗小鼠的IgG1组成。两个大鼠抗小鼠的IgG1将两个小鼠抗体联起来,其中一个小鼠抗体与需要的细胞特异结合,而另一个小鼠抗体与磁珠上包被的葡聚糖结合。当需要的细胞与磁珠结合后,在通过放在磁极上的柱子时,需要的细胞被留在柱子上,不需要的细胞会通过柱子。将留在柱子上的细胞收集起来即可进行后续的研究。 为什么选用StemSep® 正选? 1、经济,应用正选抗体 2、高纯度 3、FACS兼容










