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- Biomatik
- CAU21039
- 2025年07月14日
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文献和实验Chromatin Immunoprecipitation (ChIP)
factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic locations. In early ChIP studies, UV light from a transilluminator was used
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
, immunocytochemistry) does not automatically mean the antibody is suitable for ChIP; that has to be tested. Antibodies can be available as polyclonal or monoclonal preparations. Monoclonal antibodies have a high specificity compared to polyclonal sera
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
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