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Anti-TP53 Polyclonal Antibody

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  • ¥680 - 1600
  • 江蓝纯
  • K004192P
  • 2025年07月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      99

    • 供应商

      江蓝纯

    • 规格

      30ul/50ul/100ul

    规格:30ul产品价格:¥680.0
    规格:50ul产品价格:¥960.0
    规格:100ul产品价格:¥1600.0
    英文名称 Rabbit TP53 Polyclonal Antibody
    应用 WB,IHC,IP
    稀释比例 WB1:500-1:2000 IHC1:50-1:200 IP1:20-1:50
    交叉反应 Human,Mouse,Rat
    蛋白分子量 44kDa
    Gene ID 22059
    保存 Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.
    储存液 PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    纯化方法 Affinity purification
    亚型 IgG
    免疫原 Recombinant protein of mouse TP53
    性状 Lyophilized or Liquid
    Swiss Prot P02340
    克隆类型 Polyclonal Antibody
    来源 Rabbit
    背景资料 This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.

     

     Immunoprecipitation analysisof 150ug extracts of A549 cells  using 3ug TP53 antibody.Western blot wa sperformed from the immunoprecipitate using TP53antibody at a dilitionof 1:500.
     

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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

      , if desired this is best accomplished by Protein A Affinity Chromatography.   上一篇:make an Antibody against a Peptides   下一篇:Immunization Protocol for Monoclonal Antibody Production

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Human Anti-antibody Response

      a simple ELISA method to detect human anti-antibody response.

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