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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
spiking amount is 25 pg per well or 100 µl sample (2.5 µl of tube 2.5 to each 100 µl of sample).• DESIGNING THE PLATETo account for inter-plate variations, a complete set of standards should be included on each plate. To account for intra-plate variation
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
and some ethical issues pertaining to use of human tissues in certain countries, most studies were based on isolation and culture of cells from the lungs of small laboratory animals including mouse, rat, and rabbit. However, not much information on species
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