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文献和实验Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological states, such as normal cell turnover, embryogenesis, metamorphosis, thymic maturation and involution, neoplasm, etc. (1-4). A mouse anti-DNA monoclonal
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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