Mouse FMS Like Tyrosine Kinase

The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen

immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection

The Isolation and Identification of Murine Dendritic Cell Populations from Lymphoid Tissues and Their Production in Culture

of many of these DC subpopulations from their precursors using bone marrow cultures supplemented with fms-like tyrosine kinase 3 ligand (Flt3L). The culture-generated DC can be aligned with the populations directly isolated from tissues. Combining the in vivo

Preparation of Dendritic Cells by In Vitro Cultures

procedures encountered using ex vivo isolation protocols. Here we describe two standard protocols for in vitro differentiation of steady-state DC equivalents with Fms-like tyrosine kinase 3 ligand (Flt3L) and inflammatory-like DC using granulocyte