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- 2025年07月16日
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
Simultaneous Single‐Molecule Mapping of Protein‐DNA Interactions and DNA Methylation by MAPit
‐Schietinger, C. and Reich, N.O. 2010. The inherent processivity of the human de novo methyltransferase 3A (DNMT3A) is enhanced by DNMT3L. J. Biol. Chem. 285:29091‐29100. Hoose, S
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