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- 2025年07月07日
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文献和实验International (Cupertino, CA). BSA-NHS slides, displaying activated amino and carboxyl groups on the surface of an immobilized layer of bovine serum albumin (BSA), were fabricated as follows. 10.24 g N,N'-disuccinimidyl carbonate (100 mM) and 6.96 ml N,N
核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
temperature; add PMSF and proteinase inhibitors and put on ice before use) 5 mM PIPES (pH 8) 85 mM KCl 0.5% NP-40 1 mM PMSF proteinase inhibitors (leupeptin, aprotinin, pepstatin; each final concentration 2 µg/ml) Nuclear
HIGH RESOLUTION GENETIC FOOTPRINTING
9600 thermal cycler with thin-walled 0.2 ml tubes, and AmpliTaq polymerase from Perkin-Elmer. 6.) Materials for preparative agarose gel electrophoresis. We use the Quiaquick gel extraction kit (Qiagen) for all band excision steps, and the Quiaquick
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