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文献和实验The Generation of Multicopy Recombinant Strains
The extremely high levels of alcohol oxidase produced from the native AOX1 gene in Pichia pastoris (5–30% of cell protein on induction) suggested that single-copy AOX1 -promoter expression vectors would be sufficient for efficient foreign gene
,从 1995 年获得美国癌症学会研究奖开始,他斩获无数大奖,2015 年达到顶峰,获得美国国立卫生研究院杰出研究奖,并且当选为美国医学科学院院士。正所谓成也科研,败也科研,从 2016 年开始,陆续有人指出 Amato J. Giaccia 教授的多篇论文涉嫌造假,其中包括一篇主刊和四篇子刊,下面一起来看看这几篇文章。第一篇文章于 2006 年发表在 nature 杂志上,目前已经被引用 1202 次。《Lysyl oxidase is essential for hypoxia-induced
oxidase, derived from resting phagocytes or in the form of purified recombinant proteins, exposed to an activating agent (or situation), in the presence of NADPH and oxygen. O2 •− produced by the mixture is measured by being trapped immediately
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