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文献和实验【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
by sequence and phylogeneticanalysis. 3.1. Primer Design: Manual Method 1. Collect the protein sequences from known members of thetargeted gene family. Perform a BLAST search in the NCBIprotein database using the protein sequence of a member
was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 （英文完整版）
,which can be divided into 12 highly conserved subfamilies onthe basis of sequence similarity (6) . Many of these subfamiliesare highly conserved in early multicellular organisms such as thesea anemone, highlighting the crucial role of the Wnt pathwayin driving body
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