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文献和实验A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
with the respective MAbs, competitor MAbs, biotinylated antigen, and avidin–peroxidase conjugate. Using carcinoembryonic antigen (CEA) as a model antigen, the method was applied to determine epitope specificities of anti-CEA MAbs. A constant amount of biotinylated CEA
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
球蛋白(alpha-fetoprotein, AFP)、癌胚抗原(carcinoembryonic antigen, CEA)、胚胎性硫糖蛋白抗原(fetal sulfoslycoprotein antigen, FSA)等。胚胎抗原是最早用于肿瘤免疫学诊断和免疫学治疗的靶抗原。由于个体发育过程中对此类抗原已形成免疫耐受,故难以诱导机体产生针对胚胎抗原的杀瘤效应。 (六)过度表达的抗原 组织细胞发生癌变后,多种信号转导分子的表达量远高于正常
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