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文献和实验The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors
from a luciferase reporter have been used extensively in vitro with high-throughput screens (HTS) of large chemical compound libraries. We have generated a transgenic mouse model (CRE luc) with a luciferase reporter under the control of a synthetic promoter
Self-Inactivating Lentiviral Vectors and a Sensitive Cre-loxP Reporter System
vectors (4 ,5 ). The aim of this chapter is to introduce a SIN lentiviral vector system and the application of a sensitive Cre/loxP reporter system for vector titer determination.
, and the analysis of the quality and quantity of recombination by reporter gene and target gene studies. Most of the protocols can also be used as general guidelines for the generation and characterization of Cre/lox-mediated genome modifications in mice.
