TotalSeq™-C0360 抗人 CD357 抗体

TotalSeq™-C0360 anti-human CD357 (GITR) Antibody详细如下：

Description ：GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptorsuperfamily member (also known as AITR and TNFRSF18). GITR is expressed on activatedlymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain ofGITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation andTCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and isinvolved in the development of regulatory T cells and to regulate the activity of Th1 subsets.

Verified Reactivity ：Human
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Recombinant human GITR-Fc chimera
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application ：PG - Quality tested

Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights thatare necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, forany technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determinewhether it has all necessary third party intellectual property rights to use that platform andTotalSeq™ with that platform.

Additional Product Notes ：TotalSeq™ reagents are designed to profile protein levels at a single cell level following anoptimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10xGenomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required.Please contact technical support for more information, or visit biolegend.com/totalseq.