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- 齐源
- 109857
- 国产/进口
- 2025年07月12日
- FC
- 小鼠
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45.2
- 级别：
anti-mouse CD45.2 Antibody
- 目录编号：
anti-mouse CD45.2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-mouse CD45.2 Antibody
- 抗体英文名：
Brilliant Violet 750™ anti-mouse CD45.2 Antibody
- 抗体名：
CD45.2 抗体
- 标记物：
anti-mouse CD45.2 Antibody
- 宿主：
小鼠
- 适应物种：
小鼠
- 免疫原：
anti-mouse CD45.2 Antibody
- 亚型：
anti-mouse CD45.2 Antibody
- 形态：
anti-mouse CD45.2 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/mL
- 规格：
50 ug
Description ：CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：B10.S mouse thymocytes and splenocytes
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™under optimal conditions.
Concentration ：0.2 mg/mL
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent betitrated for optimal performance for each application.
Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm isrecommended for detection, although filter optimization may be required depending on otherfluorophores used. Be sure to verify that your cytometer configuration and software setup areappropriate for detecting this channel. Refer to your instrument manual or manufacturer forsupport. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license fromSirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use thepurchased product for research purposes only. This product may not be resold or incorporated inany manner into another product for resale. Any use for therapeutics or diagnostics is strictlyprohibited. This product is covered by U.S. Patent(s), pending patent applications and foreignequivalents.
Excitation Laser ：Violet Laser (405 nm)
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文献和实验1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
流式抗体的选择和配色原则由于在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响实验的数据质量。因此，需要考虑各种影响流式抗体品质及检测效果的因素，例如抗体特异性、荧光素信号强弱、荧光素标记方式、同型对照等。本文介绍如何选择合适的流式抗体： 1、需要满足抗体选择的最基本条件：目标蛋白特异性，反应种属以及应用实验等。 靶标特异性：确定标记物名称，确定是细胞表面表达，还是细胞内表达。细胞内表达需要进行透膜。 物种来源：确定待测样本的种属来源。例如，抗小鼠的抗体，不能用于人的样本的检测
Brilliant Blue G-250 in 50 ml of 95% ethanol. Add 100 ml of 85% phosphoric acid, and bring to a final volume of 1 liter with distilled water. Phosphoric acid is extremely corrosive. Handle with care.
