Description :Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. Itforms homodimers or heterodimers with other Bcl-2 family members. This protein regulatesapoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 hasbeen shown to enhance activity to allow response to extracellular growth-factor-mediatedsignals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin.Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed infollicular lymphomas and some diffuse large B-cell lymphomas.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :WB - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage :Each lot of this antibody is quality control tested by Western blotting. For Western blotting, thesuggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry on formalin-fixedparaffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It isrecommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicatesBioLegend citation):
1. Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC)
2. Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC)
3. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
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