- 询价
- BPS Bioscience
- 美国
- 78318
- 2025年11月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80°C (dry ice)
- 保质期：
At least 6 months at -80°C.
- 英文名：
Set of PARP Inhibitors (8 x 50 µl)
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- CAS号：
/
- 规格：
8 x 50 µl
- PARPtrap™ Assay Kits for PARP1 and for PARP2 (BPS Bioscience #80584 and #78296)
- PARPtrap™ Combo Assay Kit for PARP1 and PARP2 (BPS Bioscience #78317)
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
Immunoprecipitation of metabolically labeled cells
- dilute with Super E to convenient volume; add protease inhibitors, cold Met and antibody. Some antibodies work only after the antigen has been denatured (e.g. Ab 877, Ab 471): to 50 ul of sample in TMT, add 5 µl 10% SDS and incubate at 100 o C
Protein Expression and Purification of MBP-T antigen (131-259)
into 150 ml medium.For 6 L labeling prep.set up 2 x 150 ml of cultures into M9 labeling medium) iv.Transfer 25 ml culture into 1 L LB medium (or 50 ml of M9 culture into 1 L M9-labeling medium)containing Kan.Grow cells at 37 ℃ until the cells reach an OD
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