MART-1-Specific TCR Lentivirus

The human MART-1-specific T Cell Receptor lentiviruses (clone DMF5) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce a human TCR (clone DMF5) that specifically recognizes antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A), and in which the TCR α chain and β chain are linked by P2A (Figure 1). The lentiviruses also transduce a puromycin selection gene.  Figure 1. (A) Schematic of the lenti-vector used to generate the MART-1-specific TCR lentivirus and (B) Construct diagram showing expressed components of the MART-1-specific TCR. TRAV and TRAC correspond to the TCR alpha chain variable and constant regions, respectively, whereas TRBV and TRBC correspond to the TCR beta chain variable and constant regions.