- 询价
- BPS Bioscience
- 美国
- 101313
- 2025年09月28日
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- 技术资料
- 保存条件：
-80°C (dry ice)
- 保质期：
At least 6 months at -80°C.
- 英文名：
IL2RB, Avi-FLAG-Tag, HiP™ Recombinant
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- 规格：
100 µg
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文献和实验Recombinant Baculovirus Isolation
Although there are several different methods available of making recombinant baculovirus expression vectors (reviewed in Chapter 3), all require a stage in which insect cells are transfected with either the virus genome alone (Bac-to-Bac�
Purification of Recombinant Poly(ADP-Ribose) Polymerases
extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-aminobenzamide Sepharose™ chromatographic step. The last contaminants and the 3-methoxybenzamide used to elute the PARP from the previous
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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