- ￥1185 - 12355
- 翌圣生物（Yeasen）
- 94097ES
- 上海
- 2026年03月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Recombinant Mouse FGL2 Protein,His-Avi,Flag Tag
- 保质期：
2年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20度
- 规格：
100μg(94097ES60)/500μg(94097ES76)/25μg(94097ES25)
| 规格： | 100μg(94097ES60) | 产品价格： | ￥3525.00 |
|---|---|---|---|
| 规格： | 500μg(94097ES76) | 产品价格： | ￥12355.00 |
| 规格： | 25μg(94097ES25) | 产品价格： | ￥1185.00 |
| 表达区间及表达系统（Source） | Recombinant Mouse FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus. It contains Pro197-Pro432.[Accession | P12804] |
| 分子量大小（Molecular Weight） | The protein has a predicted MW of 31.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| 内毒素（Endotoxin） | Less than 1EU per μg by the LAL method. |
| 纯度（Purity） | > 95% as determined by Tris-Bis PAGE and HPLC |
| 制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 重构方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230721
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Analyzing the Regulation and Function of ATM
transfected cell line. ATM protein induction is monitored by immunoblotting with antibodies against both ATM and a FLAG sequence tag in the recombinant protein. Extracts from irradiated cells are immunoprecipitated with anti-ATM antibodies, and protein kinase
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