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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80°C (dry ice)
- 保质期:
At least 6 months at -80°C.
- 英文名:
IDO1, His-Avi-Tag, Biotin Labeled Recombinant
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- 规格:
50 µg
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文献和实验(Figure 2). Data generated from this assay is currently in submission. Procedure Part I: Detection of Flag-tagged proteins binding 5´ biotin labeled siRNAs in vitro 1.A minimum of ~4.0×106 cells are required/sample and clearly the more cells to begin
Comparison of Protein Expression by Isotope-Coded Affinity Tag Labeling
. The labeled proteins are then combined and digested with trypsin. The ICAT-labeled peptides are subject to the purification through an avidin column and eventually the cleavage of the biotin tags. This chapter focuses on the ICAT labeling procedure
Nonradioactive Labeling and lmmunodetection of Recombinant Proteins
, or Western blot can be carried out using enzyme-labeled primary or secondary antibodybased systems or it can be performed using biotmylated primary or secondary antibodies with streptavidin or avidin-enzyme conlugates. Biotin is often the hapten of choice
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