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- BPS Bioscience
- 美国
- 78298
- 2026年01月28日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80°C
- 保质期：
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
- 英文名：
CDK12/Cyclin K Kinase Assay Kit
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- 规格：
盒
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文献和实验2. Bösken CA, Farnung L, Hintermair C, Merzel Schachter M, Vogel-Bachmayr K, Blazek D, Anand K, Fisher RP, Eick D, Geyer M. The structure and substrate specificity of human Cdk12/Cyclin K. Nat Commun. 2014 Mar 24;5:3505. doi: 10.1038/ncomms4505. PMID: 24662513; PMCID: PMC3973122.
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Immunoprecipitation and Immune Complex kinase assay
9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10) If doing kinase assay, do additional wash in TN and then proceed with kinase assay (below). 11) Pellets can be stored dry
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
and resuspending. 10)If doing kinase assay,do additional wash in TN and then proceed with kinase assay (below). 11)Pellets can be stored dry at -20℃ for short term (one or two days)or at-70℃ for longer. 12)Resuspend pellet in at least 50 microliters sample
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