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SIRT1 (Sirtuin1) Fluorogenic A

ssay Kit
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  • 询价
  • BPS Bioscience已认证
  • 美国
  • 50081
  • 2026年01月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      -80°C

    • 保质期

      12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

    • 英文名

      SIRT1 (Sirtuin1) Fluorogenic Assay Kit

    • 库存

      1

    • 供应商

      BPS Bioscience Inc.

    • 规格

    The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling applications. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

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    图标文献和实验
    该产品被引用文献
    1. A. Ito et al. (2001) EMBO J. 20 1331.
    2. A. Ito et al. (2002) EMBO J. 21 6236.
    3. N.A. Barlev et al. (2001) Mol. Cell 8 1243. Application Reference(s):
    Middle-aged rats orally supplemented with gel-encapsulated catechin favorably increases blood cytosolic NADPH levels (2015)
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      and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples. In fact, this technique is sensitive enough to enable quantitation of RNA from a single cell. This article first discusses the advantages of real-time RT

    • 线粒体荧光探针大全:TMRM,Mitotracker,JC-1

      and of the verotoxin B subunit in Vero cells 30 Our Vybrant Apoptosis Assay Kit #11 (V35116, Section 15.5) utilizes MitoTracker CMXRos in combination with Alexa Fluor 488 annexin V in a two-color assay of apoptotic cells (Figure 15.95). MitoTracker Orange CMTMRos

    • Propagation, Quantification, Detection, and Storage of West Nile Virus

      by Quantitative Fluorogenic Reverse‐Transcription PCR Basic Protocol 4: Viral Growth Curves Alternate Protocol 1: Quantification of WNV by Focus‐Forming Assay Support Protocol 1: Culturing BHK21

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    SIRT1 (Sirtuin1) Fluorogenic Assay Kit
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