BioLegend 945101 Purified anti

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BioLegend 945101 Purified anti-SARS-CoV-2 S Protein S1
产品简介：
产品品牌：BioLegend
产品货号：945101
产品名称：BioLegend 945101 Purified anti-SARS-CoV-2 S Protein S1
产品规格：25 µg

产品说明：
Clone  A20103O (See other available formats)
Regulatory Status  RUO
Other Names  S1, Spike Protein
Isotype  Rat IgG2a, κ

描述
SARS-CoV-2是一种导致2019冠状病毒病（新冠肺炎）的呼吸道病毒。冠状病毒刺突（S）糖蛋白是病毒粒子外壳上的一种I类病毒融合蛋白，通过识别宿主细胞受体并介导病毒和细胞膜的融合，在病毒感染中发挥关键作用。S糖蛋白是由约1300个氨基酸组成的前体蛋白，然后被切割成氨基（N）末端S1亚基（约700个氨基酸）和羧基（C）末端S2亚基（600个氨基酸）。三个S1/S2异二聚体组装形成从病毒包膜突出的三聚体刺突。S1亚基包含一个受体结合结构域（RBD），该结构域可以特异性结合靶细胞上的受体血管紧张素转化酶2（ACE2）。通过受体结合、蛋白水解处理和/或细胞区室中的酸性pH触发，I类病毒融合蛋白在感染期间经历从亚稳定的融合前状态到稳定的融合后状态的转变，其中受体结合亚基被切割，融合亚基进行大规模构象重排，暴露疏水性融合肽，诱导形成六螺旋束，使病毒和细胞膜接近融合。由三个S1-S2异二聚体组成的三聚体SARS guan 状病毒（SARS-CoV-2）S糖蛋白结合细胞受体血管紧张素转化酶2（ACE2），并通过融合前到融合后的构象转变介导病毒和细胞膜的融合。
SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.

Product Details
Verified Reactivity   SARS-CoV-2
Antibody Type   Monoclonal
Host Species  Rat
Immunogen
Partial recombinant SARS-CoV-2 S protein corresponding to S1 subunit

Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration   0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application   WB - Quality tested
Direct ELISA, FC - Verified

Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For Direct ELISA, a concentration of 114.9 ng/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.

Antigen Details
Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits.

Distribution
Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Mediates fusion between virus and host cell membranes
Interaction   ACE2
Ligand/Receptor   ACE2
Biology Area   COVID-19

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