Sigma货号217669抗CD28小鼠单克隆抗体(ANC2

Sigma货号217669抗CD28小鼠单克隆抗体(ANC28.1/5D10)上海睿安生物13611631389

Sigma货号217669现货Anti-CD28 Mouse mAb(ANC28.1/5D10)上海睿安生物13611631389

说明

一般描述

在人T细胞和转染了人CD28的BHK细胞上识别CD28。

小鼠抗CD28单克隆抗体(克隆ANC28.1/5D10)识别人T细胞上的CD28 & 人CD28转染的BHK细胞。 已针对冰冻切片的FC，IP，IHC和T细胞共刺激进行了验证。 
蛋白A纯化的小鼠单克隆抗体。识别CD28蛋白。

免疫原

人
人CD28 Ig融合蛋白和HPB-MLT人肿瘤细胞

应用

流式细胞术（5 µg/ml）

冷冻切片（参见评论）

免疫沉淀法（参见评论）

T 细胞共刺激（参见评论）

包装:请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外形:在150mM NaCl，100mM KCl，50mM磷酸钠中，pH 7.5。

重悬:初次使用后，分装冻存(-20°C)以进行长期储存。避免冻/融循环。

其他说明

Kariv, I., et al. 1996.J. Immunol.157, 29.
61st Forum in Immunology.1995.Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G.1993.Curr.Opin.Immunol.5, 361.
单体和交联抗体会刺激 CD28+ 细胞表达 IL-2。使用 5 µg/ml 的抗 CD28 抗体和 FITC 偶联的山羊抗小鼠 IgG 对 5x10⁵ 培养的 HPB-MLT 细胞进行 FACS。也有报道表明该抗体可用于冷冻切片和免疫沉淀。合适的工作稀释度由与测定条件相关的变量决定。

法律信息:CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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