描述 阿尔茨海默病的特征是聚集的Aβ肽在老年斑块和血管沉积物中积聚。Aβ肽来源于淀粉样蛋白前体蛋白(APP),通过β-分泌酶和γ-分泌酶对APP的顺序蛋白水解切割产生不同的Aβ种类。β可以聚集形成可溶性寡聚物和不溶性原纤维或无定形组装体。某些形式的聚集肽对神经元是有毒的。 Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
Product Details Verified Reactivity Human Antibody Type Monoclonal Host Species Mouse Preparation Ascites Concentration The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers. Application WB - Quality tested Direct ELISA, IHC-P – Verified IHC-F, EM - Reported in the literature, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details Structure Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation Molecular Family APP/β-Amyloid
以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 100711 APC anti-mouse CD8a 53-6.7 25 ug BioLegend 100712 APC anti-mouse CD8a 53-6.7 100 ug BioLegend 100713 APC/Cyanine7 anti-mouse CD8a 53-6.7 25 ug BioLegend 100714 APC/Cyanine7 anti-mouse CD8a 53-6.7 100 ug BioLegend 100721 PE/Cyanine7 anti-mouse CD8a 53-6.7 25 ug BioLegend 100723 Alexa Fluor® 488 anti-mouse CD8a 53-6.7 100 ug BioLegend 100724 Alexa Fluor® 647 anti-mouse CD8a 53-6.7 100 ug BioLegend 100725 Pacific Blue™ anti-mouse CD8a 53-6.7 100 ug BioLegend 100726 Alexa Fluor® 488 anti-mouse CD8a 53-6.7 25 ug BioLegend 100727 Alexa Fluor® 647 anti-mouse CD8a 53-6.7 25 ug BioLegend 100728 Pacific Blue™ anti-mouse CD8a 53-6.7 25 ug BioLegend 100730 Alexa Fluor® 700 anti-mouse CD8a 53-6.7 100 ug BioLegend 100731 PerCP anti-mouse CD8a 53-6.7 25 ug BioLegend 100733 PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 25 ug BioLegend 100734 PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 100 ug BioLegend 100741 Brilliant Violet 650™ anti-mouse CD8a 53-6.7 125 uL BioLegend 100744 Brilliant Violet 605™ anti-mouse CD8a 53-6.7 50 ug BioLegend 100747 Brilliant Violet 711™ anti-mouse CD8a 53-6.7 125 uL BioLegend 100748 Brilliant Violet 711™ anti-mouse CD8a 53-6.7 500 uL BioLegend 100749 Brilliant Violet 785™ anti-mouse CD8a 53-6.7 125 uL BioLegend 100751 Brilliant Violet 510™ anti-mouse CD8a 53-6.7 125 uL BioLegend 100752 Brilliant Violet 510™ anti-mouse CD8a 53-6.7 50 ug