BioLegend 681307 PE anti-SPI1

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BioLegend 681307 PE anti-SPI1 (PU.1)
产品简介：
产品品牌：BioLegend
产品货号：681307
产品名称：BioLegend 681307 PE anti-SPI1 (PU.1)
产品规格：25 µg

产品说明：
Clone   7C2C34 (See other available formats)
Regulatory Status   RUO
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
Isotype   Rat IgG2a, κ

描述
SPI1是一种属于E26转化特异性（ETS）家族的转录因子，仅在造血细胞中表达。SPI1在造血干细胞分化过程中调节细胞命运决定，这对淋巴和骨髓细胞谱系的发育至关重要。SPI1缺陷小鼠缺乏巨噬细胞、中性粒细胞和B淋巴细胞，它们在出生前或出生后不久死亡。SPI1表达异常可导致发育缺陷和恶性肿瘤。SPI1的过表达阻断红系分化并抑制细胞死亡。携带突变SPI1等位基因的小鼠表现出SPI1表达降低并发展为急性髓细胞白血病（AML），这表明SPI1在肿瘤发生中起作用。
SPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.

Product Details
Isotype Control   PE Rat IgG2a, κ Isotype Ctrl
Verified Reactivity   Mouse
Antibody Type   Monoclonal
Host Species   Rat
Immunogen
Full length SPI1 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration   0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application   ICFC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details
Structure
272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
Distribution   Nucleus.

Function
SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.

Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family   Nuclear Markers

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